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中文名(CN) 英文名(EN) CAS No. 规格(SP) 报价(CNY) 产地(From) 类别(Key)
胶原酶 IV (四型) Collagenase from Clostridium histolyticum (Type IV) 9001-12-1 100MG 620.00 Sigma-C5138 酶和辅酶
胶原酶 IV (四型) Collagenase from Clostridium histolyticum (Type IV) 9001-12-1 1G 3780.00 Sigma-C5138 酶和辅酶

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中文名称 胶原酶 IV (四型)
英文名称 Collagenase from Clostridium histolyticum (Type IV)
CAS识别号 9001-12-1
MDL识别号 MFCD00130830
EC识别号 232-582-9
Beilstein Null,without related properties.
分子式 Null,without related properties.
分子量 Null,without related properties.
分子结构图 分子图
产品性状 Null,without related properties.
质量标准 Molecular Weight: This collagenase obtained from the culture filtrate of Clostridium histolyticum. The culture filtrate that is obtained is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.1
Many references exist for using collagenase to digest various tissues. The choice of one technique over another is often arbitrary and based more on past experience than on an understanding of why the method works and what modifications could lead to better results. Concentrations typically vary from 0.1 to 5 mg/ml, and digestion time is something that should be experimentally monitored using a very gentle agitation system to check for tissue dissociation. Collagenase treatment can cause some cells to die. Satisfactory efficiency of cell dissociation without causing too much cell death typically is achieved from 15 minutes to several hours, but can fall
outside of this range if the concentration is unusual. The preferred buffer to use is Krebs Ringer Buffer with calcium and BSA. Zn2+ is required for activity, but it is tightly bound to the collagenase during purification. Additional Zn2+ should not be necessary as long as no chelator is added to the solution during digestion.
储存条件 -20 °C 冷藏保存,Stored at -20 °C
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