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中文名(CN) 英文名(EN) CAS No. 规格(SP) 报价(CNY) 产地(From) 类别(Key)
dNTPs Deoxynucleotide Mix (10 mM, ≥99%) Null 0.5ML 230.00 Sigma-D7295 核酸衍生物

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中文名称 dNTPs
英文名称 Deoxynucleotide Mix (10 mM, ≥99%)
CAS识别号 Null
MDL识别号 Null,without related properties.
EC识别号 Null,without related properties.
Beilstein Null,without related properties.
分子式 Null,without related properties.
分子量 Null,without related properties.
分子结构图 分子图
产品性状 Null,without related properties.
质量标准 PCR Suitability
dNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing10 mM Trizma?-HCl, pH 8.3 at 25 °C, 50 mM KCl, 1.5 mM MgCl2,
0.001% (w/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng/100 μl, and Taq DNA polymerase at 2.5 units/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments, and 72 °C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel.
储存条件 -20 °C 冷藏保存,Stored at -20 °C
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